Characterization of l-2-keto-3-deoxyfuconate aldolases in a nonphosphorylating l-fucose metabolism pathway in anaerobic bacteria
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منابع مشابه
Identification and Characterization of L - Arabonate Dehydratase , L - 2 - Keto - 3 - deoxyarabonate Dehydratase , and L - Arabinolactonase Involved in an Alternative Pathway of L - Arabinose Metabolism
From the Faculty of Engineering, Kyoto University, Kyotodaigaku-katsura, Saikyo-ku, Kyoto 615-8530, Japan, the Institute of Advanced Energy, Kyoto University, Gokasyo, Uji, Kyoto 611-0011, Japan, the Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan, and CREST, JST (Japan Science and Technology Agency), Gokasyo, Uji, Kyoto 611-00...
متن کاملThe Metabolism of L - Fucose
The isomerization of L-fucose to L-fuculose was observed by Green and Cohen (2) in extracts obtained from Escherichia coli grown on L-fucose. With the same organism and similar conditions, Huang and Miller (3) suggested that such extracts catalyzed the phosphorylation of both L-fucose and L-fuculose to form the corresponding sugar l-phosphates. The present report describes the partial purificat...
متن کاملMetabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation.
L-Lactaldehyde is a branching point in the metabolic pathway of L-fucose and L-rhamnose utilization. Under aerobic conditions, L-lactaldehyde is oxidized to L-lactate by the enzyme lactaldehyde dehydrogenase, while under anaerobic conditions, L-lactaldehyde is reduced to L-1,2-propanediol by the enzyme propanediol oxidoreductase. Aerobic growth on either of the methyl pentoses induces a lactald...
متن کاملPreliminary crystallographic analysis of l-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of l-arabinose metabolism
L-2-Keto-3-deoxyarabonate (L-KDA) dehydratase is a novel member of the dihydrodipicolinate synthase (DHDPS)/N-acetylneuraminate lyase (NAL) protein family and catalyzes the hydration of L-KDA to alpha-ketoglutaric semialdehyde. L-KDA dehydratase was overexpressed, purified and crystallized at 291 K using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.0 A resolution using s...
متن کاملL-Ascorbic acid degradation by bacteria. VIII. All the pathway in the L-ascorbic acid metabolism.
The medium used contained 3g of substrate, 150ml of 1/15M phosphate buffer and 150ml of sterile distilled water. 0.5g (dry weight) of the adapted cells was added to the medium and the whole was incubated at 30•‹ for 20 hours. For detecting the intermediate products, paper chromatography was chiefly used. For developing the nonvolatile acids, n-butanol-acetic acid-water (4:1:1) was used and the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2019
ISSN: 0021-9258,1083-351X
DOI: 10.1074/jbc.ra119.011854